For the purification of peptides, insulin (analogues) and oligonucleotides, it is often difficult to use methods similar to those applied in the purification of other organic compounds, mainly due to their complexity. Purification methods usually utilize various principles of chromatography such as ion exchange chromatography and medium or high-pressure reversed-phase chromatography.
Due to increasing government and public pressure to decrease costs of medicines, the purification process should be as simple as possible and contain a minimum of steps. However, the reality is that by using today's classical ion-exchange and/or reversed-phase purification methods, multiple steps are still required to achieve the desired purity. Of course, this has a huge impact on the recovery (yield). Besides this, more organic solvent must be used – all resulting in an inefficient, costly purification process.
ZEOsphere DRP Mixed-Mode materials can substantially improve the selectivity of charged molecules from the adsorption on the hydrophobic sites and repulsion on the charged sites.
This enhanced selectivity leads to desired purity more quickly and will considerably increase the recovery (yield). Because ZEOsphere DRP Mixed-Mode materials can work with low organic solvent conditions, a decrease in organic solvent usage is obtained.
ZEOsphere DRP Mixed-Mode products are today’s answer to improve compound purification and to considerably lower production costs.